abstract：INTRODUCTION:The Mindray CAL 8000 is a cellular analysis line that consists of the BC-6800, an automated hematology analyzer, and the SC-120, an automated slidemaker/stainer. We evaluated the performances of the BC-6800 and the SC-120. METHODS:Four hundred and eight normal and abnormal samples were analyzed. The perfo...

journal_title：Journal of clinical laboratory analysis

authors： Lee HT,Park PW,Seo YH,Kim KH,Seo JY,Jeong JH,Kim MJ,Ahn JY

更新日期：2017-07-01 00:00:00

abstract：BACKGROUND:The association between genetic variations of vascular endothelial growth factor (VEGF) gene and the risk for atherosclerosis has been hypothesized. We aimed to assess the relationship between rs2010963 (+405 C/G) polymorphism and presence, severity, and outcome of coronary artery disease (CAD) in an Iranian...

journal_title：Journal of clinical laboratory analysis

authors： Kalayi Nia S,Ziaee S,Boroumand MA,Sotudeh Anvari M,Pourgholi L,Jalali A

更新日期：2017-07-01 00:00:00

abstract：BACKGROUND:In this study, the fresh stool samples from 254 children under 5 years of age with acute gastroenteritis which were delivered between October 2012 and December 2013 were collected. METHODS:In the stool samples, rotavirus antigens were investigated using two different immunochromatographic methods which are ...

journal_title：Journal of clinical laboratory analysis

authors： Artiran S,Atalay A,Gökahmetoglu S,Ozturk MA,Balci N,Cakir N,Kilic H,Durmaz R

更新日期：2017-03-01 00:00:00

abstract：BACKGROUND:Essential trace elements are vital to human health. In this study, our aim was to establish reference intervals of and to evaluate relationships among Ca, Fe, Cu, Mg, and Zn for children. METHODS:We collected blood samples of 3210 children aged 0-14 years from Lu'an, China, and concentrations of the above e...

journal_title：Journal of clinical laboratory analysis

authors： Zhai R,Zhang M,Liu J,Guang H,Li B,Chen D,Zhang S

更新日期：2017-03-01 00:00:00

abstract：BACKGROUND:Dried blood spots (DBS) are an important form of bio-sampling and valuable approach for storing blood samples for genetic studies. This has necessitated in developing an effective protocol to isolate genomic DNA (gDNA) from DBS samples.In this study, we have elucidated a dependable and non-hazardous "single ...

journal_title：Journal of clinical laboratory analysis

authors： Shaik M,Shivanna DK,Kamate M,Ab V,Tp KV

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:Hypoalbuminemia has been proven to be a powerful predictor of mortality in adult patients. However, prognostic value of serum albumin in neonates is not clear. OBJECTIVE:To assess the relationship between serum albumin level within the first day of life and outcome in preterm infants born before 32 weeks of...

journal_title：Journal of clinical laboratory analysis

authors： Torer B,Hanta D,Yapakci E,Gokmen Z,Parlakgumus A,Gulcan H,Tarcan A

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:Neuron-specific enolase (NSE) is a recognized biomarker for the assessment of cerebral injury in neurological disorders. This study aims to report a definitive assessment of the biological variation (BV) components of this biomarker, including within-subject BV (CVI), between-subject BV (CVG), index of indiv...

journal_title：Journal of clinical laboratory analysis

authors： Matyar S,Goruroglu Ozturk O,Ziyanoglu Karacor E,Yuzbasioglu Ariyurek S,Sahin G,Kibar F,Yaman A,Inal T

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:Miltenberger (Mi) series are the collective glycophorin hybrids in the MNS blood group system. Mi series are composed of several subtypes, for examples, GP.Mur, GP.Hop, and GP.Bun. The incompatibility of Mi series blood transfusion poses the risk of hemolysis. Due to the lack of standard antibodies for Mi se...

journal_title：Journal of clinical laboratory analysis

authors： Vongsakulyanon A,Pipatpanukul C,Kitpoka P,Kunakorn M,Srikhirin T

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:Risperidone (RIS) is a widely used atypical antipsychotic drug. We developed and validated a sensitive and accurate LC-MS/MS method, which requires a small-volume of plasma and small-volume injection for measurement of RIS levels in ASD pediatric patients. We also investigated the relationship between RIS le...

journal_title：Journal of clinical laboratory analysis

authors： Vanwong N,Prommas S,Puangpetch A,Hongkaew Y,Nuntamool N,Nakorn CN,Ngamsamut N,Limsila P,Sukasem C

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray ...

journal_title：Journal of clinical laboratory analysis

authors： Ciepiela O,Kotuła I,Kierat S,Sieczkowska S,Podsiadłowska A,Jenczelewska A,Księżarczyk K,Demkow U

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:Lupus nephritis is a serious manifestation of systemic lupus erythematosus (SLE). The objective of this study was to identify the sensitivity, specificity, and cut-off values of IP-10 in the serum and urine of patients with lupus nephritis compared to renal biopsy, albumin/creatinine ratio, and serum anti-ds...

journal_title：Journal of clinical laboratory analysis

authors： El-Gohary A,Hegazy A,Abbas M,Kamel N,Nasef SI

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:DRI cotinine assay is suitable only for screening for cotinine in urine specimens. We studied the reliability of DRI cotinine semiquantitative values by comparing them with the cotinine concentration obtained with a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. METHODS:Semiquantitative c...

journal_title：Journal of clinical laboratory analysis

authors： Dixon RB,Dasgupta A

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND & AIM:Hepatitis B virus-e-antigen (HBeAg) is an affordable viral marker to assess viral replication kinetics and response to antiviral therapy. In the absence of confirmatory assays, discrepant or false-positive HBeAg results are resolved by screening for other HBV markers. We standardized an in-house HBeAg ...

journal_title：Journal of clinical laboratory analysis

authors： Fletcher GJ,Anantharam R,Radhakrishnan K,Karunakaran A,Abraham P

更新日期：2016-11-01 00:00:00

abstract：BACKGROUNDS:β-Thalassemia is one of the most prevalent worldwide autosomal recessive disorders. It presents a great molecular heterogeneity resulting from more than 200 causative mutations in the β-globin gene. In Tunisia, β-thalassemia represents the most prevalent monogenic hemoglobin disorder with 2.21% of carriers....

journal_title：Journal of clinical laboratory analysis

authors： Sahli CA,Ben Salem I,Jouini L,Laouini N,Dabboubi R,Hadj Fredj S,Siala H,Othmeni R,Dakhlaoui B,Fattoum S,Bibi A,Messaoud T

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:This study was aimed to investigate whether measurement of free testosterone and cortisol in saliva is a reliable alternative to their assessment in serum for monitoring physical fitness in professional athletes. METHODS:We studied 25 members of the soccer team Parma F.C., playing in Italian major football ...

journal_title：Journal of clinical laboratory analysis

authors： Lippi G,Dipalo M,Buonocore R,Gnocchi C,Aloe R,Delsignore R

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:The aim of this study was to detect reliability of Brucella Coombs gel test (BCGT) by comparing with with ELISA (IgG + IgM), Standard agglutination test, and Brucella immunocapture agglutination methods in serological diagnosis of brucellosis. METHODS:Brucella Coombs gel test (BCGT), Brucella ELISA (IgG + I...

journal_title：Journal of clinical laboratory analysis

authors： Kalem F,Ergün AG,Durmaz S,Doğan M,Ertuğrul Ö,Gündem S

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:The human epididymis protein 4 (HE4) may have high specificity in the detection of malignant diseases, making the development of an immunoassay for HE4 essential. METHODS:In our study, a fusion gene was constructed encoded with the HE4 protein. This protein was then produced in the bacterial cells (Escheric...

journal_title：Journal of clinical laboratory analysis

authors： Zhou L,Lv Z,Shao J,Xu Y,Luo X,Zhang Y,Hu Y,Zhang W,Luo S,Fang J,Wang Y,Duan C,Huang R

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:We evaluated the new body fluid module on Sysmex UF1000-i (UF1000i-BF) for analysis of white blood cell (WBC) and red blood cell (RBC) in cerebrospinal fluid (CSF). METHODS:WBC and RBC counting were compared between UF1000i-BF and Fuchs-Rosenthal counting chamber in 67 CSF samples. This study also included ...

journal_title：Journal of clinical laboratory analysis

authors： Buoro S,Apassiti Esposito S,Alessio M,Crippa A,Ottomano C,Lippi G

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:Definitive detection of hemoglobin (Hb) variants requires DNA sequencing. High-resolution melting (HRM) analysis of polymerase chain reaction (PCR) amplicons was applied to detect and discriminate among uncommon α-Hb variants found in Thailand. METHODS:Uncommon suspected α-Hb variants observed in Hb typing ...

journal_title：Journal of clinical laboratory analysis

authors： Yimniam W,Jindadamrongwech S

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:Toxocariasis is the clinical term that is applied to infection in the human host with Toxocara species larvae. Serological tests are important tools for the diagnosis of toxocariasis. The aim of this study was to evaluate the excretory-secretory (ES) antigens of T. cati larvae using enzyme-linked immunosorbe...

journal_title：Journal of clinical laboratory analysis

authors： Zibaei M,Sadjjadi SM,Sarkari B,Uga S

更新日期：2016-05-01 00:00:00

abstract：BACKGROUND:A convenient biosensor for real-time measurement of biomarkers for in-field psychophysiological stress research and military operations is desirable. We evaluated a hand-held device for measuring salivary amylase as a stress marker in medical technicians undergoing combat casualty care training using two dif...

journal_title：Journal of clinical laboratory analysis

authors： Peng HT,Savage E,Vartanian O,Smith S,Rhind SG,Tenn C,Bjamason S

更新日期：2016-05-01 00:00:00

abstract：BACKGROUND:CD4(+) cell count, the common HIV infection screening test, is costly and unable to differentiate HIV monoinfection from its concurrent infection with hepatitis B or C virus. We aimed to ascertain diagnostic value of serum adenosine deaminase (ADA) activity as a useful tool to differentiate HIV mono- and co-...

journal_title：Journal of clinical laboratory analysis

authors： Abdi M,Rahbari R,Khatooni Z,Naseri N,Najafi A,Khodadadi I

更新日期：2016-05-01 00:00:00

abstract：BACKGROUND:This study was performed to investigate the epidemiology of enterovirus (EV) infections in children at a single hospital during recent 5 years. This study was preformed from December 2008 to November 2013. METHODS:We subjected 1,067 cerebrospinal fluid (CSF) specimens from 1,053 patients and 678 stool speci...

journal_title：Journal of clinical laboratory analysis

authors： Jeon JS,Kim JK,Yu J,Kim JW

更新日期：2016-03-01 00:00:00

abstract：BACKGROUND:Today, the genetic and genomic research entered in a new era of high-throughput genotyping technology. However, mutagenic polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is still a choice of genotyping method in molecular epidemiological research. It has been extensively used fo...

journal_title：Journal of clinical laboratory analysis

authors： Sharma N,Awasthi S,Phadke SR

更新日期：2016-03-01 00:00:00

abstract：BACKGROUND:This study aimed to investigate the relationship between HbA1c and RBC aggregation in type 2 diabetes mellitus (T2DM) patients by analysis of data from routine clinical tests and from in vitro experiments. METHODS:A total of 2,111 inpatients with type 2 diabetes were selected and among them, 364 patients (G...

journal_title：Journal of clinical laboratory analysis

authors： Li Q,Li L,Li Y

更新日期：2015-09-01 00:00:00

abstract：OBJECTIVES:Existing evidence suggests that impaired vitamin D metabolism contribute to the development of atherosclerosis. Aortic intima-media thickness (IMT) is an earlier marker than carotid IMT of preclinical atherosclerosis. However, there is a lack of researches on direct investigation of relevance between serum 2...

journal_title：Journal of clinical laboratory analysis

authors： Kalkan GY,Gür M,Koyunsever NY,Şeker T,Gözükara MY,Uçar H,Kaypaklı O,Baykan AO,Akyol S,Türkoğlu C,Elbasan Z,Şahin DY,Çaylı M

更新日期：2015-07-01 00:00:00

abstract：BACKGROUND:Platelet-reactive antibodies lead to thrombocytopenia and bleeding disorders, and diverse assays are used for their detection. In this retrospective analysis, the applicability of three different test systems was compared and antibody specificities were assessed. METHODS:Sera of 1,234 patients were tested w...

journal_title：Journal of clinical laboratory analysis

authors： Sareban N,Macher S,Drexler C,Posch U,Lanzer G,Schallmoser K

更新日期：2015-05-01 00:00:00

abstract：BACKGROUND:Although it is widely acknowledged that physical activity confers several health benefits, it remains uncertain whether strenuous and physically demanding exercise might determine biological effects that might turn to be ultimately unfavorable for health. Copeptin and midregion proadrenomedullin (MR-proADM) ...

journal_title：Journal of clinical laboratory analysis

authors： Lippi G,Schena F,Salvagno GL,Sanchis-Gomar F,Guidi GC

更新日期：2015-01-01 00:00:00

abstract：AIM:To evaluate, retrospectively, the frequency of autoantibodies of antiphospholipid syndrome (APLS) in Tunisian patients with primary biliary cirrhosis (PBC). PATIENTS AND METHODS:We analyzed 80 PBC sera and 80 sera from blood donors. ELISA was used to determine the frequency of antibodies against cardiolipin (aCL I...

journal_title：Journal of clinical laboratory analysis

authors： Mankaï A,Manoubi W,Ghozzi M,Melayah S,Sakly W,Ghedira I

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Reference intervals are important for interpretation of clinical laboratory tests. The platelet (PLT) indices such as the mean platelet volume (MPV) and platelet distribution width (PDW) are newer hematological parameters, which have been recently reported as clinically valuable biomarkers. However, there ar...

journal_title：Journal of clinical laboratory analysis

authors： Hong J,Min Z,Bai-shen P,Jie Z,Ming-ting P,Xian-zhang H,Xiao-ke H,Lan-lan W,Xin Z,Wei G,Rui Q,Wen-xiang C,Xin-zhong W,Yue-yun M,Hong S

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Kawasaki disease (KD) is an acute, self-limited vasculitis in infants and young children. Interleukin-10 (IL-10) is a potent cytokine that exerts pleiotropic effects on immunoregulation and inflammation. Elevated IL-10 serum levels have been reported in the KD patients. METHODS:In this study, we investigate...

journal_title：Journal of clinical laboratory analysis

authors： Lin YJ,Lan YC,Lai CH,Lin TH,Huang SM,Liao CC,Lin CW,Hung CH,Tien N,Liu X,Chien WK,Chen JH,Tsai FJ

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND:The sialylation of serum proteins and lipids changes in liver diseases of different etiologies and could change the total sialic acid (TSA), lipid-bound SA (LSA), and free SA (FSA) levels in the sera. However, little is known of the relationship of serum SAs concentrations and the severity of liver disease. ...

journal_title：Journal of clinical laboratory analysis

authors： Chrostek L,Supronowicz L,Panasiuk A,Cylwik B,Gruszewska E,Szmitkowski M

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND:After hematopoietic stem cell transplantation (HSCT), early detection of engraftment would be of critical value for clinicians. The aim of this study was to identify faster parameters for engraftment. METHODS:We evaluated blood cell parameters including complete blood count (CBC), differential counts, and v...

journal_title：Journal of clinical laboratory analysis

authors： Yahng SA,Lee JW,Kim Y,Kim M,Oh EJ,Park YJ,Lee JW,Cho B,Han K

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND:Recent studies have shown that neutrophil lymphocyte ratio (NLR) is a strong indicator in determining inflammation in cardiac and non-cardiac diseases. We aimed to evaluate the relationship between proteinuria and NLR in chronic kidney disease (CKD) patients without diabetes mellitus (DM). METHODS:Between 2...

journal_title：Journal of clinical laboratory analysis

authors： Binnetoğlu E,Şengül E,Halhallı G,Dindar S,Şen H

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND:Nonalcoholic fatty liver disease (NAFLD) is an emerging problem all over the world. Because NAFLD and polycystic ovary syndrome (PCOS) are both closely related with insulin resistance, it would be necessary to determine the rate of presence of NAFLD in PCOS patients. So, this study aimed to investigate the u...

journal_title：Journal of clinical laboratory analysis

authors： Caner S,Altınbaş A,Saykı M,Büyükcam F,Yılmaz B,Çakal E,Çoban Ş,Delibaşı T

更新日期：2014-09-01 00:00:00

abstract：BACKGROUND:For the forensic aim, a sensitive and specific method using headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in blood using n-propanol as internal standard. GC was performed in isothermal mode with a GC run-time of 5.0 min. ...

journal_title：Journal of clinical laboratory analysis

authors： Xiao HT,He L,Tong RS,Yu JY,Chen L,Zou J,Li JQ,Bian Y,Zhang Y

更新日期：2014-09-01 00:00:00

abstract：BACKGROUND:Iron overload is a major complication in patients with hemoglobin H (Hb H) disease and causes damage of tissues. METHODS:We investigated 26 Hb H patients and 75 controls to evaluate their oxidative stress and antioxidant statuses. RESULTS:There were significantly increased levels of superoxide anion in leu...

journal_title：Journal of clinical laboratory analysis

authors： Chiou SS,Tsao CJ,Tsai SM,Wu YR,Liao YM,Lin PC,Tsai LY

更新日期：2014-07-01 00:00:00

abstract：BACKGROUND:Lupus nephritis is one of the most serious manifestations of systemic lupus erythematosus (SLE). Novel biomarkers are necessary to enhance the diagnostic accuracy, prognostic stratification, monitoring of treatment response, and detection of early renal flares. METHODS:Our study was conducted on 90 particip...

journal_title：Journal of clinical laboratory analysis

authors： Mohammed MF,Belal D,Bakry S,Marie MA,Rashed L,Eldin RE,El-Hamid SA

更新日期：2014-07-01 00:00:00

abstract：BACKGROUND:Identification of dermatophytes at the species level, relying on macro- and microscopic properties of the colonies is time-consuming, questioned in many circumstances, and requires considerable expertise. In this study, we examined the potency of a new genetic marker, β-tubulin (BT2) gene, for differentiatio...

journal_title：Journal of clinical laboratory analysis

authors： Abastabar M,Mirhendi H,Rezaei-Matehkolaei A,Shidfar MR,Kordbacheh P,Makimura K

更新日期：2014-03-01 00:00:00

abstract：AIM:To investigate the presence of antipituitary antibodies (APA) in the serum of patients undergoing gastroenteropathies (GEP) other than celiac disease (CD). METHODS:APA were analyzed in GEP patients (n = 103), CD patients (n = 94), idiopathic growth hormone (GH) deficiency patients (n = 21), and 98 age- and sex-mat...

journal_title：Journal of clinical laboratory analysis

authors： Aguado R,Fernández S,Estévez OA,Santamaría M,Ortega C

更新日期：2014-01-01 00:00:00